Screening for Biosurfactant-Producing Bacteria Isolated from Abattoir Soil
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Abstract
Biosurfactants possess essential properties that make them highly indispensable in many industries, such as waste management, food, agriculture, and pharmaceuticals, due to their surface-active and wetting abilities. The search for microorganisms capable of producing this extensively utilized biomolecule is increasing daily. In this study, abattoir soil collected from Awka metropolis, Anambra State, was screened for biosurfactant-producing bacteria using mineral salt medium (MSM) supplemented with engine oil. Three bacterial isolates (NJ1, NJ2, and NJ3) were obtained from MSM agar. These isolates were subjected to four biosurfactant screening assays: haemolysis test, drop collapse test, oil spreading test, and emulsification index (E24). The results indicated that all three isolates showed alpha-haemolytic activity, and drop collapse positive reactions. Notably, NJ3 exhibited complete collapse reaction (+++) in the drop collapse test. Additionally, in the oil spreading test, NJ3 demonstrated the highest displacement of engine oil (10 mm), followed by NJ2 (7 mm) and NJ1 (5 mm). The determination of E24 using kerosene for all isolates revealed that NJ1 exhibited the highest E24 at 13%, while NJ2 and NJ3 had E24 of 2.56% and 2.50%, respectively. Morphological characterization, Gram’s staining, and biochemical analyses performed on the isolates, identified NJ1 and NJ3 as members of Streptomyces spp., while NJ2 was identified as Bacillus sp. These findings suggest that abattoir soil could be a potential source of biosurfactant-producing bacteria.
Biosurfactants possess essential properties that make them highly indispensable in many industries, such as waste management, food, agriculture, and pharmaceuticals, due to their surface-active and wetting abilities. The search for microorganisms capable of producing this extensively utilised biomolecule is increasing daily. In this study, abattoir soil collected from Awka metropolis, Anambra State, was screened for biosurfactant-producing bacteria using mineral salt medium (MSM) supplemented with engine oil. Three bacterial isolates (NJ1, NJ2, and NJ3) were obtained from MSM agar. These isolates were subjected to four biosurfactant screening assays: haemolysis test, drop collapse test, oil spreading test, and emulsification index (E24). The results indicated that all three isolates showed alpha-haemolytic activity and drop collapse positive reactions. Notably, NJ3 exhibited a complete collapse reaction (+++) in the drop collapse test. Additionally, in the oil spreading test, NJ3 demonstrated the highest displacement of engine oil (10 mm), followed by NJ2 (7 mm) and NJ1 (5 mm). The determination of E24 using kerosene for all isolates revealed that NJ1 exhibited the highest E24 at 13%, while NJ2 and NJ3 had E24 of 2.56% and 2.50%, respectively. Morphological characterisation, Gram staining, and biochemical analyses performed on the isolates identified NJ1 and NJ3 as members of Streptomyces spp., while NJ2 was identified as Bacillus sp. These findings suggest that abattoir soil could be a potential source of biosurfactant-producing bacteria.
Keywords: Biosurfactant, Abattoir, Oil Spreading, Screening, Streptomyces spp
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This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain copyright of their work, with first publication rights granted to Fountain Journal of Natural and Applied Sciences. Articles in FUJNAS are published on the Creative Commons Attribution 4.0 International license (CC BY 4.0).
Miriam Adaku Okpara, Nnamdi Azikiwe University, Awka
Department of Applied Microbiology and Brewing
Anulika Goodness Chukwujekwu, Nnamdi Azikiwe University, Awka
Department of Applied Microbiology and Brewing
References
Alves, A. R., Sequeira, A. M. & Cunha, A. (2019). Increase in bacterial biosurfactant production by co-cultivation with biofilm-forming bacteria. Letters in Applied Microbiology 69, 79—86.
Ambaye, T. G., Vaccari, M., Prasad, S. & Rtimi, S. (2021). Preparation, characterization and application of biosurfactant in various industries: A critical review on progress, challenges and perspectives. Environmental Technology and Innovation 24, 102090.
Astuti, D. I., Purwasena, I. A., Putri, R. E., Amaniyah, M., & Sugai, Y. (2019). Screening and characterization of biosurfactant produced by Pseudoxanthomonas sp. G3 and its applicability for enhanced oil recovery. Journal of Petroleum Exploration and Production Technology 9, 2279–2289 https://doi.org/10.1007/s13202-019-0619-8.
Bicca, F. C., Fleck, L. C. & Ayub, M. A. Z. (1999). Production of biosurfactant by hydrocarbon degrading Rhodococcus erythropolis. Environmental Microbiology 30, 1—3.
Biniarz, P., ?ukaszewicz, M. & Janek, T. (2015). Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review. Critical Reviews in Biotechnology http://dx.doi.org/10.3109/07388551.2016.1163324.
Bjerk, T. R., Severino, P., Jain, S., Marques, C., Silva, A. M., Pashirova, T. & Souto, E. B. (2021). Biosurfactants: properties and applications in drug delivery, Biotechnology and Ecotoxicology. Bioengineering 8, 115 https://doi.org/10.3390/bioengineering8080115.
Bodour, A. A. & Miller-Maier, R. M. (1998). Application of a modified drop collapse technique for surfactant quantification and screening of biosurfactant-producing microorganisms. Journal of Microbiology Methods 32, 273–280.
Cai, Q., Zhang, B., Chen, B., Song, X., Zhu, Z. & Cao, T. (2015). Screening of biosurfactant-producing bacteria from offshore oil and gas platforms in North Atlantic Canada. Environmental Monitoring Assessment 187, 284 https://doi.org/10.1007/s10661-015-4490-x.
da Silva, G. F., Gautam, A., Duarte, I. C. S., Delforno, T. P. , de Oliveira, V. M. & Huson, D. H. (2022). Interactive analysis of biosurfactants in fruit waste fermentation samples using BioSurfDB and MEGAN. Scientific Reports 12, 7769.
Derguine Mecheri, L., Kebbouche Gana, S., & Djenane, D. (2021). Biosurfactant production from newly isolated Rhodotorula sp.YBR and its great potential in enhanced removal of hydrocarbons from contaminated soils. World Journal of Microbiology and Biotechnology 37, 18. https://doi.org/10.1007/s11274-020-02983-3.
Dwivedi, A., Kumar, A. & Bhat, J. L. (2019). Production and characterization of biosurfactant from Corynebacterium species and its effect on the growth of petroleum degrading bacteria. Microbiology 88(1), 87–93.
Holt, S. G., Kriey, N. R., Sneath, P. H. A., Staley, J.T. & Williams, S.T. (1993). Bergey’s’s manual of determinative for bacteriology New York: Williams and Wilkins.
Iqbal, S., Khalid, Z. M. & Malik, K. A. (1995). Enhanced biodegradation and emulsification of crude oil and hyperproduction of biosurfactants by gamma ray-induced mutant of Pseudomonas aeruginosa. Letter in Applied Microbiology 21, 176—179.
Jain, D., Collins-Thompson, D. & Lee, H. (1991). A drop-collapseing test for screening surfactant-producing microorganisms. Journal of Microbiological Methods 13(4), 271—279.
John, W. C., Ogbonna, I. O., Gberikon, G. M. & Iheukwumere, C. C. (2020). Screening and characterization of biosurfactant-producing Bacillus species isolated from contaminated soils in Makurdi metropolis. Nigerian Journal of Biotechnology 37(2), 165-176.
Marchut Miko?ajczyk, O., Dro?d?y?ski, P., Polewczyk, A., Smu?ek, W. & Antczak, T. (2021). Biosurfactant from endophytic Bacillus pumilus 2A: physicochemical characterization, production and optimization and potential for plant growth promotion. Microbial Cell Factories 20, 40.
Martinho, V., Lima, L. M. d-S., Barros, C. A., Ferrari, V. B., Passarini, M. R. Z., Santos, L. A., Sebastianes, F. L. d-S., Lacava, P. T. & de Vasconcellos, S. P. (2019). Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil. AMB Express 9, 130.
Morikawa, M., Hirata, Y. & Imanaka, T., (2000). A study on the structure-function relationship of the lipopeptide biosurfactants. Biochimica et Biophysica Acta 1488, 211–218.
Ndibe, T. O., Eugene, W. C. & Usman, J. J. (2018). Screening of Biosurfactant-Producing Bacteria Isolated from River Rido, Kaduna, Nigeria. Journal of Applied Science and Environmental Management 22(11), 1855–1861.
Nikolova, C. & Gutierrez, T. (2021). Biosurfactants and their applications in the oil and gas industry: Current state of knowledge and future perspectives. Frontiers in Bioengineering and Biotechnology 9, 626639.
Nunes, H. M. A. R., Vieira, I. M. M., Santos, B. L. P., Silva, D. P. & Ruzene, D. S. (2021). Biosurfactants produced from corncob: a bibliometric perspective of a renewable and promising substrate. Preparative Biochemistry & Biotechnology https://doi.org/10.1080/10826068.2021.1929319.
Pardhi, D., Panchal, R. & Rajput, K. (2020). Screening of biosurfactant producing bacteria and optimization of production conditions for Pseudomonas guguanensis D30, Bioscience Biotechnology Research Communication 13 (1), 171—179.
Pardhi, D. S., Panchal, R. R., Raval, V. H., Joshi, R. G., Poczai, P., Almalki, W. H. & Rajput, K. N. (2022). Microbial surfactants: A journey from fundamentals to recent advances. Frontiers in Microbiology 13, 982603. https://doi.org/ 10.3389/fmicb.2022.982603.
Patowary K., Patowary, R., Kalita, M. C. & Deka, S. (2017). Characterization of biosurfactant produced during degradation of hydrocarbons using crude oil as sole source of carbon. Frontiers in Microbiology 8, 279 https://doi.org/10.3389/fmicb.2017.00279
Raj, A., Kumar, A. & Dames, J. F. (2021). Tapping the role of microbial biosurfactants in pesticide remediation: An eco-friendly approach for environmental sustainability. Frontiers in Microbiology 12, 791723 https://doi.org/10.3389/fmicb.2021.791723.
Rani M., Weadge, J. T. & Jabaji, S. (2020) Isolation and characterization of biosurfactant-producing bacteria from oil well batteries with antimicrobial activities against food-borne and plant pathogens. Frontiers in Microbiology 11, 64 https://doi.org/10.3389/fmicb.2020.00064.
Ruangprachaya, F. & Chuenchomrat, P. (2018). Isolation and Characterization of Biosurfactant Produced by Lactic Acid Bacteria from Indigenous Thai Fermented Foods. International Journal of Food Engineering 4(4), 268—272.
Samsu, Z. A., Jeffry, F. N. & Azizan, W. N. A. N. W. A. R. (2020). Isolation and screening of potential biosurfactant-producing bacteria from used engine oil-contaminated soil. Materials Today: Proceedings 31, A67–A71.
Santos, D. K. F., Rufino, R. D., Luna, J. M., Santos, V. A. & Sarubbo, L. A. (2016). Biosurfactants: multifunctional biomolecules of the 21st Century. International Journal of Molecular Sciences 17, 401¬—431.
Satpute, S. K., Bhawsar, B. D., Dhakephalkar, P. K., & Chopade, B. A. (2008). Assessment of different screening methods for selecting biosurfactant-producing marine bacteria. Indian Journal of Marine Science 37(3), 243–250.
Satpute, S. K., Banpurkar, A. G., Dhakephalkar, P. K., Banat, I. M. & Chopade, B. A. (2010). Methods for investigating biosurfactants and bioemulsifiers: a review. Critical Reviews in Biotechnology 30(2), 127–144.
Sharma, D., Ansari, M. J., Al-Ghamdi, A., Adgaba, N., Khan, K. A., Pruthi, V. & Al-Waili, N. (2015). Biosurfactant production by Pseudomonas aeruginosa DSVP20 isolated from petroleum hydrocarbon-contaminated soil and its physicochemical characterization. Environmental Science and Pollution Research https://doi.org/10.1007/s11356-015-4937-1.
Silva, M. E. T., Duvoisin Jr, S., Oliveira, R. L., Banhos, E. F., Souza, A. Q. L. & Albuquerque, P. M. (2019). Biosurfactant production of Piper hispidum endophytic fungi. Journal of Applied Microbiology https://doi.org/10.1111/jam.14398.
Sohail, R. & Jamil, N. (2020). Isolation of biosurfactant producing bacteria from Potwar oil fields: Effect of non-fossil fuel based carbon sources. Green Process Synthesis 9, 77–86.
Uyar & Saglam (2021). Isolation, screening and molecular characterization of biosurfactant producing bacteria from soil samples of auto repair shops. Archives of Microbiology https://doi.org/10.1007/s00203-021-02483-4.
Uzoigwe C., Burgess, J. G., Ennis, C. J., & Rahman, P. K. S. M. (2015). Bioemulsifiers are not biosurfactants and require different screening approaches. Frontiers in Microbiology 6, 245. http://doi: 10.3389/fmicb.2015.00245.
Walter, V., Syldatk, C., & Hausman, R. (2010) Screening concepts for the isolation of biosurfactant producing microorganisms. In: R. Sen (ed.) Biosurfactants. Advances in Experimental Medicine and Biology, vol 672. New York, NY: Springer. https://doi.org/10.1007/978-1-4419-5979-9_1.
Youssef, N. H., Duncana, K. E., Naglea, D. P., Savagea, K. N., Knappb, R. M. & McInerney, M. J. (2004). Comparison of methods to detect biosurfactant production by diverse microorganisms. Journal of Microbiological Methods 56, 339 – 347.