Purification of Wickerhamomyces anomalus Keratinase and Its Prospective Application in Poultry Feed Industries

Authors

  • Ayandiran Aina Babcock University
  • C. O Ezeamagu Department of Microbiology, Babcock University, Ilishan-Remo, Ogun State, Nigeria.
  • S. T. Akindele Department of Microbiology, Babcock University, Ilishan-Remo, Ogun State, Nigeria.
  • A. O. Aleshinloye Chemistry Unit, Department of Basic Sciences, Babcock University, Ilishan-Remo, Ogun State, Nigeria.

DOI:

https://doi.org/10.53704/fujnas.v10i1.345

Abstract

Animal wastes emanating from cow horns, hooves and feathers are keratinous in nature which can only be degraded by keratinolytic microorganisms. Consequently, the pollution resulting from the accumulation of these wastes in response to growing livestock demand is posing a significant threat to human health and the environment. This study was carried out using cow hooves as the substrate for the production of keratinase from fungal identified as Wickerhamomyces anomalus 9 (18S rDNA gene sequencing) was isolated from soil rich hooves using basal salt agar medium and potato dextrose agar. The keratinase of the isolate was assessed using skim milk agar and the enzyme was produced by solid-state fermentation.  The crude enzyme was purified using ammonium sulphate precipitation, ion exchange and gel-filtration chromatography. The specific activity of the enzyme was 0.29 U/mg with a yield of 45% and a 7.25 purification fold. The optimal pH and temperature of the enzyme were 8.0 and 60 oC respectively. The enzyme was observed to be thermo-stable at 50oC between for 30 minutes. The kinetics revealed that the Vmax was 0.384U/min with Km 86.95mg/ml. The native molecular weight of the enzyme was found to be 34KDa. There were significant differences at 95% confidence with poultry feed treated with Wickerhamomyces anomalus keratinase in moisture, ash content, crude fibre, crude fat, nitrogen content, crude protein and carbohydrate compare to the untreated feed. These results suggest an environment-friendly approach for biodegradation of cow hooves wastes for the production of keratinases, animal waste management as well as a promising tool for chicken feed additives.

Keywords: Biodegradation, Chromatography, Cow hooves, Keratinase, Pollution

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Published

2021-04-21

How to Cite

Aina, A., Ezeamagu, C. O., Akindele, S. T., & Aleshinloye, A. O. (2021). Purification of Wickerhamomyces anomalus Keratinase and Its Prospective Application in Poultry Feed Industries. Fountain Journal of Natural and Applied Sciences, 10(1). https://doi.org/10.53704/fujnas.v10i1.345