Phytochemical Content, Radical Scavenging and Antibacterial Properties of Aqueous Extract of Jatropha curcas Linn Leaves

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O. B. Oloyede
A. K. Salau
R. T. Akewusola
O. T. Ganiyu
L. A. Azeez
S. M. Ogunbode


This study investigated phytochemical content, radical scavenging and antibacterial activities of aqueous extract of leaves of Jatropha curcas Linn. Quantitative phytochemical analyses of alkaloids, phenols, tannins and flavonoids contents were carried out; radical scavenging activity was assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and hydrogen peroxide inhibition assays while Disc diffusion and Agar well (ditch) diffusion methods were used for antibacterial activity against Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, Staphilococcus aureus and Proteus species at 62.5, 125, 250 and 500 mg/mL. The extract contained alkaloids (1.600 ± 0.58 %), tannins (0.121 ± 0.00 mg/mL), phenols (0.463 ± 0.06 mg/mL) and flavonoids (0.672 ± 0.00 mg/mL), and showed radical scavenging activities against DPPH (IC50 = 21.24) and hydrogen peroxide (15.67 mg/mL) which were less than that of Butylated hydroxyanisole (BHA); IC50 = 3.92 (DPPH) and 6.19 mg/mL (hydrogen peroxide) respectively. It also showed antibacterial activity against Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa at 250 and 500 mg/ml (MIC = 125 mg/mL), which were resistant to ampicillin, chloxacillin and erythromycin, and the extract was inactive against Staphilococcus aureus and Proteus species at these concentrations, though all were sensitive to gentamycin. This shows that the aqueous extract of leaves of Jatropha curcas Linn may possess some of the folkloric properties claimed.

Keywords: Jatropha curcas; Euphorbiaceae; aqueous extract; antibacterial activity; radical scavenging activity


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Oloyede, O. B., Salau, A. K., Akewusola, R. T., Ganiyu, O. T., Azeez, L. A., & Ogunbode, S. M. (2012). Phytochemical Content, Radical Scavenging and Antibacterial Properties of Aqueous Extract of Jatropha curcas Linn Leaves. Fountain Journal of Natural and Applied Sciences, 1(1).


Ajboye, T. O., Yakubu, M. T., Salau, A. K., Oladiji, A. T., Akanji, M. A. and Okogun, J. I. (2010). Antioxidant and drug detoxification potential of aqueous extract of Annona senegalensis leaves in carbon tetrachloride-induced hepatocellular damage. Pharmaceutical Biology 48(12), 1361-1370.

Belewu, M. A. (2008). Replacement of fungus treated Jatropha kernel meal for soybean meal in the diet of rats. Green Farming 2(3), 154-157.

Belewu, M. A. and Ogunsola, F. O. (2010). Haematological and serum indices of goat fed fungi treated Jatropha curcas kernel cake in a mixed ration. Journal of Agricultural Biotechnology and Sustainable Development 2(3), 35-38.

Belewu, M. A., Adekola, F. A., Adebayo, G. B., Ameen, O. M., Muhammed, N. O., Olaniyan, A. M., Adekola, O. F. and Musa, A. K. (2010). Physico-chemical characteristics of oil and biodiesel from Nigerian and Indian Jatropha curcas seeds. International Journal of Biological and Chemical Science 4(2), 524-529.

Cheesebrough, M. (2000). District Laboratory Practice in Tropical countries. Part 1, 1st edition. press Syndicate of the University of Cambridge, pp 132-143.

Ejelonu, B. C., Oderinde, R. A. and Balogun, S. A. (2010). Chemical and Biological Properties of Jatropha curcas and Mucuna solan Seed and Seed Oil. Libyan Agriculture Research Center Journal International 1(4), 263-268.

El Diwani, G., El Rafie, S. and Hawash, S. (2009). Antioxidant activity of extracts obtained from residues of nodes leaves stem and root of Egyptian Jatropha curcas. African Journal of Pharmacy and Pharmacology 3(11), 521-530.

Gajendiran, N. and Mahadevan, A. (1990). Plasmidborne catechol dissimilation in Rhizobium sp. FEMS Microbiology Letters 73, 125-130.

Gbolade, A. A. (2009). Inventory of antidiabetic plants in selected districts of Lagos State, Nigeria. Journal of Ethnopharmacology 121, 135-139.

Harborne, J. B. (1973). Phytochemical Methods. A guide to modern techniques of plant analysis. London. New York. Chapman and Hall.

He, J., Chen, L., Heber, D., Shi, W. and Lu, O. Y. (2006) Antibacterial compounds from Glycyrrhiza uralensis. Journal of Natural Product 69(1), 121-124.

Igbinosa, O. O, Igbinosa, E. O. and Aiyegoro, O. A. (2009). Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). African Journal of Pharmacy and Pharmacology 3(2), 58-62.

Jagadish, L. K., Krishnan, V. V., Shenbha, G. R. and Kaviyasan, V. (2009). Comparative study on the antioxidant, anticancer, and antimicrobial property of Agaricus bisporus imbach before and after boiling. African Journal of Biotechnology 8, 654- 661.

James, O., Unekwojo, E. G. and Ojochenemi, A. A. (2011). Assessment of Biological Activities: A Comparison of Pergularia daemia and Jatropha curcas Leaf Extracts. British Biotechnology Journal 1(3), 85-100.

Kalimuthu, K. Vijayakumar, S. and Senthilkumar, R. (2010). Antimicrobial activity of the biodiesel plant, Jatropha curcas L. International Journal of Pharma and Bio Sciences 1(3), 1-5.

Kamal, S., Manmohan, S. and Birendra, S. (2011). A review on chemical and medicobiological applications of Jatropha curcas. International Research Journal of Pharmacy, 61-66.

Meyer, J. J. M., Afolayan, A. J., Taylor, M. B. and Erasmus, D. (1997). Antiviral activity of galangin from the aerial parts of Helichrysum aureonitens. Journal of Ethanopharmacology 56, 165-169.

Min, B. R., Pinchak, W. E., Merkel, R., Walker, S., Tomita, G. and Anderson, R. C. (2008). Comparative antimicrobial activity of tannin extracts from perennial plants on mastitis pathogens. Scientific Research and Essay 3(2), 066-073.

Nair, R. and Chando, S. A. (2005). Anticandidal activity of Punica granatum exhibited in different solvents. Pharmacol. Biol., 42: 21-25.

Namuli, A., Abdullah, N., Sieo, C. C., Zuhainis, S. W. and Oskoueian, E. (2011). Phytochemical compounds and antibacterial activity of Jatropha curcas Linn extracts. Journal of Medicinal Plants Research 5(16), 3982-3990.

Okujagu, T. F., Etatuvie, S. O, Eze, I., Jimoh, B., Nweke, C. and Mbaoji, C. (2006). Medicinal Plants of Nigeria; South-west Nigeria. In collaboration with Lagos state Traditional Medicine Board and Botany Department, University of Lagos. 1st ed. 1: 20.

Olajire, A. A. and Azeez, L. (2011). Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. African Journal of Food Science and Technology 2, 22-29.

Oseni, L. A. and Alphonse, P. K. (2011). Comparison of antibacterial properties of solvent extracts of different parts of Jatropha curcas (Linn). International Journal of Pharmaceutical and Phytopharmacological Research 1(3), 117-123.

Oskoueian, E., Abdullah, N., Saad, W. Z., Omar, A., Ahmad, S., Kuan, W. B., Zolkifli, N. A., Hendra, R. and Ho, Y. W. (2011). Antioxidant, antiinflammatory and anticancer activities of methanolic extracts from Jatropha curcas Linn. Journal of Medicinal Plants Research 5(1), 49-57.

Potchoo, Y., Guissou, I. P., Lompo, M., Sakie, E. and Yaro, B. (2008a). Antioxidant activity of aqueous, methanol and ethyl acetate extract of leaves of Annona senegalensis Pers. From Togo versus the one originates from Burkina Faso. International Journal of Pharmacology 4, 120-124.

Potchoo, Y., Richard, D., Sakie, E., Guissou, I. P., Kini, F. and Yaro, B. (2008b). Comparative phytochemical content of leaves extracts of two Annona senegalensis Pers.: The one from Togo and the other originates from Burkina Faso. Journal of Biological Science 8, 577-583.

Rathee, P., Chaudhary, H., Rathee, S., Rathee, D., Kumar, V. and Kohli, K. (2009). Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflammation and Allergy Drug Targets 8(3), 229-235.

Rug, M. & Ruppel, A. (2000). Toxic activities of the plant Jatropha curcas against intermediate snail hosts and larvae of Schistosomes. Tropical Medicine & International Health 5, 423-430.

Thomas, R., Sah, N. and Sharma, P. (2008). Therapeutic Biology of Jatropha curcas: A Mini Review. Current Pharmaceutical Biotechnology 9(4), 315-324.

Turkoglu, A., Duru, M. E., Mercan, N., Kivrak, I. and Gezer, K. (2007) Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry 101, 267-273.

Uche, F. I & Aprioku, J. S. (2008) The Phytochemical Constituents, Analgesic and Anti-inflammatory effects of methanol extract of Jatropha curcas leaves in Mice and Wister albino rats. Jounal of Applied Science and Environmental Management 12(4), 99-102.

Upadhyay, R. K. (2011) Plant natural products: Their pharmaceutical potential against disease and drug resistant microbial pathogens. Journal of Pharmacy Research 4(4), 11791185.