Antibiotic Susceptibility Patterns of Pseudomonas Species Isolated From Clinical and Environmental Samples in Awba Dam, Ibadan

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T. Y. Senbadejo
O. E. Fagade

Abstract

Pseudomonas species are common pathogenic Gram negative bacteria frequently found in both environmental and clinical samples. They are opportunistic pathogen and could be multidrug resistant (MDR); their presence in the environment signifies threat to the public health. Water from Awba dam is used as municipal water supply for drinking, fishing and agricultural purposes in the University of Ibadan which gives reason for proper monitoring. The study was designed to isolate and characterize Pseudomonas species present in Awba dam reservoir and also to compare its antimicrobial resistance pattern with clinical isolates. Pseudomonas was isolated using Centrimide agar (Oxoid) and identification was done to species level using classical method. Disk diffusion method was applied to test antibiotic sensitivity according to clinical and laboratory standard guidelines. A total of 200 bacteria isolates were obtained from water and sediment, of which 30 were found positive for Pseudomonas giving a prevalence of 15.0% (30/200). Eighteen clinical samples were collected from University College Hospital with about 50% from urine, 27.8% (Ear), 11.1% (wound) and 5.6% each from pleural effusion and nasopharyngeal tube. Two species of Pseudomonas; aeruginosa and fluorescens were identified. Out of the 10 antibiotics used, environmental isolates showed no resistance to gentamicin, amikacin, ciprofloxacin, tetracycline and imipinem while clinical isolates showed 83.3, 16.7, 83.3, 100 and 11.1% resistance to the antibiotics respectively. Amikacin and Imipinem was the most effective drug for both environmental and clinical Pseudomonas isolates. High rates of MDR Pseudomonas (94.4%) from clinical strains and 13.3% of the environmental strains were observed. In conclusion, the result shows that Pseudomonas isolates from the clinical specimens are more exposed to antibiotics, and MDR isolates in the water system can be transferred to humans and animals.


Keywords: Antibiotic resistance, water, multidrug resistance (MDR), Pseudomonas aeruginosa, Pseudomonas fluorescens

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Senbadejo, T. Y., & Fagade, O. E. (2017). Antibiotic Susceptibility Patterns of Pseudomonas Species Isolated From Clinical and Environmental Samples in Awba Dam, Ibadan. Fountain Journal of Natural and Applied Sciences, 6(2). https://doi.org/10.53704/fujnas.v6i2.149
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References

Alexopoulos, A., Plessas, S., Voidarou, C., Noussias, H., Stavropoulou, E., Mantzourani, I., Tzora, A., Skoufos, I. and Bezirtzoglou, E. (2011). Microbial ecology of fish species growing in Greek sea farms and their watery environment. Anaerobe. (A paper on the microbial ecology of fishes)

Brooks, G.F., Karen, C.C., Janet, S.B. and Stephen, A.M. (2011). Medical Microbiology. Jawetz, Melnick, & Adelberg. 25th edition, McGraw-Hill LANG. Chapter 16.

Campisano, A., Overhage, J. and Rehm, B.H. (2008). The polyhydroxyalkanoate biosynthesis genes are differentially regulated in planktonic- and biofilm-grown Pseudomonas aeruginosa. Biotechnology. 133: 442-452.

Iwane, T., Urase, T. and Yamamoto, K. (2001). Possible impact of treated wastewater discharge on incidence of antibiotic resistant bacteria in river water. Water Science Technology. 43: 91-99.

Jalal, S. and Wretlind, B. (1998). Mechanisms of quinolone resistance in clinical strains of Pseudomonas aeruginosa. Microbiology Drug Resistance. 4: 257–261.

Magiorakos, A. P. (2011). Multidrug- Resistant (MDR), Extensively Drug Resistant (XDR) and Pandrug-1 Resistant (PDR) Bacteria in Healthcare Settings. Expert Proposal for a Standardized International Terminology.

Marufa, N., Animesh, S., Malek, M.A. and Ansaruzzaman, M.D. (2015). Prevalence and resistance pattern of Pseudomonas aeruginosa isolated from surface water. Advances in Microbiology. 5: 74-81.

Mavridou, A., Papapetropoulou, M., Boufa, P., Lambiri, M. and Papadakis, J.A. (1994). Microbiological quality of bottled water in Greece. Letters in Applied Microbiology. 19: 213–216.

Meenakumari, S., Verma, S,H., Absar, A. and Chaudhary, A. (2011). Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa in an Indian cardiac hospital. International Journal Engineering Science and Technology 3:7117-7124.

Mena, K.D. and Gerba, C.P. (2009). Risk assessment of Pseudomonas aeruginosa in water. Reviews of Environmental Contamination and Toxicology 201: 71–115.

Murray, P.R., Rosenthal, K.S., Kobayashi, G.S. and Pfaller, M.A. (2002). Medical Microbiology. Fourth Edition. Philadelphia: Elsevier Mosby, pp. 278-303.

Navon-Venezia, S., Ben-Ami, R. and Carmeli, Y. (2005) Update on Pseudomonas aeruginosa and Acinetobacter baumannii infections in the healthcare setting. Current Opinion on Infectious Diseases. 18: 306–313.

Oduyebo O.O., Ogunsola, F.T. and Odugbemi, T. (1997). Prevalence of multi-resistant strains of P.aeruginosa isolated at the Lagos University Teaching Hospital from 1994- 1996. Nigeria Quarterly Journal of Medicine. 7: 373-376.

Okeke, I.N. and Sosa, A. (2003). Antibiotic resistance in Africa – Discerning the enemy and plotting a defence. African Health. 25(3); 10-15.29

Olayinka, B.O., Olonitola, O.S. and Olayinka, A.T. and Agada, E. A. (2004). Antibiotic susceptibility pattern and multiple antibiotic resistance index of Pseudomonas aeruginosa urine isolates from a University Teaching Hospital. African Journal of Clinical and Experimental Microbiology. 5 (2): 198-202.

Olayinka, A.T., Onile, B.A. and Olayinka, B.O. (2009). Antibiotic susceptibility and plasmid pattern of Pseudomonas aeruginosa from the surgical unit of a university teaching hospital in north central Nigeria. Internal Journal of Medicine and Medical Sciences. 1(3): 079-083.

Pirnay J.-P., Matthijs S., Colak H., Chablain P., Bilocq F., van Eldere J., de Vos D., Zizi M., Triest L. and Cornelis, P. (2005). Global Pseudomonas aeruginosa biodiversity as reflected in a Belgian river. Environmental Microbiology. 7:969–980.

Rossolini,G.A. and Mantengoli, E. (2005). Clinical Microbiology and Infection. Science Direct. 11: 17 - 32

Schwartz, T., Kohnen, W., Jansen, B. and Obst, U. (2003). Detection of antibiotic-resistant bacteria and their resistance genes in wastewater, surface water, and drinking water biofilms. FEMS Microbiology Ecology. 43: 325–335.

Silby, M.W., Winstanley, C., Godfrey, S.A.C., Levy, S.B. and Jackson, R.W. (2011). Pseudomonas genomes: diverse and adaptable, FEMS Microbiology Reviews. 35: 652 – 680.

Todar, K. (2011). Pseudomonas species. Todar’s online book of bacteriology.