Synergistic Ameliorative Capabilities of Quercetin and Ascorbic Acid on Hepatic and Pro-Inflammatory Markers of Arsenic-Induced Toxicity in Obese Wistar Rats

Authors

  • Adeola Busayo Adenmosun Osun State University
  • Omowumi Oyeronke Adewale Osun State University
  • Tope G. Atere Osun State University
  • Olu Israel Oyewole Osun State University

DOI:

https://doi.org/10.53704/fujnas.v13i2.509

Keywords:

Body Mass Index, Dysfunctions, Quercetin, Ascorbic acid, Obesity, Arsenic

Abstract

Obesity and arsenic exposure have been linked to many health issues. However, arsenic poisoning severity and susceptibility may depend on Body Mass Index (BMI), oxidative state, food supplements, and other factors. This study examines how quercetin and ascorbic acid can improve arsenic-induced health dysfunctions in normal and high BMI male Wistar rats. A total of 70 male Wistar rats weighing between 135 - 220 g were divided into 10 groups. A: Control group; B: sodium arsenite group; C: quercetin + sodium arsenite group; D: ascorbic acid + sodium arsenite group; E: sodium arsenite + quercetin + ascorbic acid; F: obese group; G: obese + sodium arsenite group; H: obese + sodium arsenite + quercetin group; I: obese + sodium arsenite + ascorbic acid group; J: obese + sodium arsenite + quercetin + ascorbic acid group, all treatments were administered orally daily for twenty-eight (28) days. The doses administered were 595 mg/kg body weight of ascorbic acid (10 % LD50), 50 mg/kg body weight of quercetin (5 % LD50), and 0.75 mg/kg body weight of sodium arsenite (5 % LD50). Concentrations of interleukin-1β (IL-1β), interleukin 6 (IL 6), TNF-alpha (TNF-α), COX-2, nitric oxide (NO), total bilirubin (TBL) and activities of alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT), and gamma-glutamyl transferase (GGT) were determined in plasma. Upon the completion of the experiment, serum IL-1β, IL 6, TNF-α, COX-2, NO, TBL concentrations and ALP, AST, ALT, and GGT activities increased significantly (p<0.05) across the groups and treatment with quercetin and ascorbic acid ameliorated the pathological alteration elicited or caused by sodium arsenite. The findings showed that arsenic exposure altered pro-inflammatory and hepatic function indices in high BMI rats, while treatment with quercetin and ascorbic acid exhibited an ameliorative effect against this obesity and arsenic-induced health dysfunctions.


Keywords: Body Mass Index, Dysfunctions, Sodium arsenite, Inflammation

References

Aasheim, E.T., Hofso, D., Hjelmesaeth, J., Birkeland, K.I., and Bohmer. T. (2008). Vitamin status in morbidly obese patients: a cross-sectional study. America Journal Clinical Nutrition. (87):362-369.

Abdelkarem, H.M. and Fadda, L.H. (2013). Flaxseed and quercetin improve anti-inflammatory cytokine level and insulin sensitivity in animal model of metabolic syndrome, the fructose-fed rats. Arabian Journal of Chemistry 3(2); 331.341.

Adewale O.O., Ekundayo S.S., Oluwuyi O.O., Akomolafe S.F., (2018). Co-exposure of lead acetate and sodium arsenite causes alteration in the markers of liver and kidney functions in male Wistar rats. Journal of Chemical, Environmental and Biological Engineering 2(1); 32-39.

Afolabi, O., Oyewo, E., Adekunle, A., Adedosu, O., & Adedeji, A. (2013). Assessment of Lipid Peroxidation Markers and Proinflammatory Cytokines in Arsenite-Exposed Rats. Assessment, 2, 8-18.

Black, P. H. (2003). The inflammatory response is an integral part of the stress response: Implications for atherosclerosis, insulin resistance, type II diabetes and metabolic syndrome X. Brain, Behavior, and Immunity, 17(5), 350-364.

Canoy, D., Wareham, N., Welch, A., Bingham, S., Luben, R., Day, N., and Khaw. K.T. (2005). Plasma ascorbic acid concentrations and fat distribution in 19,068 British men and women in the European prospective investigation into cancer and nutrition Norfolk cohort study. American Journal Clinical Nutrition. 82(60):1203-1209.

Castriota, F., Acevedo, J., Ferreccio, C., Smith, A. H., Liaw, J., Smith, M.T., Steinmaus, C., (2018). Obesity and increased susceptibility to arsenic-related type 2 diabetes in Northern Chile. Environ. Res. 167, 248–254.

Chen, L., Li, J., Luo, C., Liu, H., Xu, W., Chen, G., & Jiang, H. (2006). Binding interaction of quercetin-3-?-galactoside and its synthetic derivatives with SARS-CoV 3CLpro: Structure– activity relationship studies reveal salient pharmacophore features. Bioorganic & medicinal chemistry, 14(24), 8295-8306

Chiang, L. C., Chiang, W., Liu, M. C., & Lin, C. C. (2003). In vitro antiviral activities of Caesalpinia pulcherrima and its related flavonoids. Journal of Antimicrobial Chemotherapy, 52(2), 194-198.

?íž, M., Pavelkova, M., Gallova, L., Kralova, J., Kubala, L., & Lojek, A. (2008). The influence of wine polyphenols on reactive oxygen and nitrogen species production by murine macrophages RAW 264.7. Physiological Research, 57(3), 393-402.

Costa M., (2019). Review of arsenic toxicity, speciation and polyadenylation of canonical histones. Toxicol Appl Pharmacol.,15;375:1-4.

Coussens, L. M., & Werb, Z. (2002). Inflammation and cancer. Nature, 420(6917), 860-867.

Cushnie, T. T., & Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26(5), 343-356.

Das, N., Paul, S., Chatterjee, D., Banerjee, N., Majumder, N. S., Sarma, N., and Giri, A. K., (2012). Arsenic exposure through drinking water increases the risk of liver and cardiovascular diseases in the population of West Bengal, India. BMC Public Health, 12, 639.

Davis, J. M., Murphy, E. A., McClellan, J. L., Carmichael, M. D., & Gangemi, J. D. (2008). Quercetin reduces susceptibility to influenza infection following stressful exercise. America Journal Physiol Regul Integr Comp Physiol, 295, R505-R509.

De Pergola G, Silvestris F. (2013). Obesity as a major risk factor for cancer. J Obes.:291546-52.

Diego F.G., Patricia L., Pablo Q., Jose A.M., (2014). Vitamin C in the Treatment and/or Prevention of Obesity [Review]. Journal of Nutritional Science and vitaminology. 60(6):367-379.

Erdman Jr, J. W., Balentine, D., Arab, L., Beecher, G., Dwyer, J. T., Folts, J., & Burrowes, J. (2007). Flavonoids and heart health: proceedings of the ILSI North America flavonoids workshop, May 31–June 1, 2005, Washington, DC. The Journal of Nutrition, 137(3), 718S-737S.

Gomez-Rubio, P., Roberge, J., Arendell, L., Harris, R. B., O'Rourke, M. K., Chen, Z., & Klimecki, W. T. (2011). Association between body mass index and arsenic methylation efficiency in adult women from southwest US and northwest Mexico. Toxicology and applied pharmacology, 252(2), 176-182.

Houben, T., Brandsma, E., Walenbergh, S. M. A., Hofker, M. H., & ShiriSverdlov, R. (2017). Oxidized LDL at the crossroads of immunity in non-alcoholic steatohepatitis. Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids, 1862(4), 416-429.

Hughes, M. F., Beck, B. D., Chen, Y., Lewis, A. S., & Thomas, D. J. (2011). Arsenic exposure and toxicology: a historical perspective. Toxicological sciences, 123(2), 305-332.

Jomova, K., Jenisova, Z., Feszterova, M., Baros, S., Liska, J., Hudecova, D., and Valko, M. (2011). Arsenic: toxicity, oxidative stress and human disease. Journal of Applied Toxicology, 31(2), 95107.

Leemans, J. C., Kors, L., Anders, H. J., & Florquin, S. (2014). Pattern recognition receptors and the inflammasome in kidney disease. Nature Reviews Nephrology, 10(7), 398-414.

Mandal, B. K., & Suzuki, K. T. (2002). Arsenic round the world: a review. Talanta, 58(1), 201-235. 82.

Maria E.G., Lisa V., Nihit B., Andrew W., and Sophia R., (2022). Cytokines IL-4, IL-13, and IL-10 Promote Differentiation of Pro-Lymphatic Progenitors Derived from Bone Marrow Myeloid Precursors. Stem Cells and Development. 322-3

Marseglia, L.; Manti, S.; D’Angelo, G.; Nicotera, A.; Parisi, E.; Di Rosa, G.; Gitto, E.; Arrigo, T. (2015). Oxidative Stress in Obesity: A Critical Component in Human Diseases. International Journal. Molecular. Science, 16: 378-400.

Maulucci, G., Daniel, B., Cohen, D., Avrahami, Y., & Sasson, S. (2016). Hormetic and regulatory effects of lipid peroxidation mediators in pancreatic beta cells. Molecular Aspects of Medicine, 49, 49-77.

Medrano, M. J., Boix, R., Pastor-Barriuso, R., Palau, M., Damián, J., Ramis, R., and NavasAcien, A. (2010). Arsenic in public water supplies and cardiovascular mortality in Spain. Environmental Research, 110(5), 448-454.

Moon, K. A., Guallar, E., Umans, J. G., Devereux, R. B., Best, L. G., Francesconi, K. A., and NavasAcien, A. (2013). Association between exposure to low to moderate arsenic levels and incident cardiovascular disease: a prospective cohort study. Annals of Internal Medicine, 159(10), 649 - 659.

Nair M.P., Mahajan S, Reynolds J.L., Aalinkeel R, Nair H, Schwartz S.A., and Kandaswami C: (2006). The flavonoid quercetin inhibits proinflammatory cytokine (tumor necrosis factor-alpha) gene expression in normal peripheral blood mononuclear cells via modulation of the NF?kappa beta system. Clin Vaccine Immunol 13: 319?328.

National Research Council. (2014). Critical Aspects of EPA’s IRIS Assessment of Inorganic Arsenic: Interim Report. Washington, DC: The National Academies Press.

Naujokas M. F., Anderson B., Ahsan H., Aposhian H. V., Graziano J. H., Thompson C., Suk W. A (2013). The broad scope of health effects from chronic arsenic exposure; update on a worldwide public health problem. Environ Health Perspect 121 (3); 295-302.

Neuhouser, M. L. (2004). Dietary flavonoids and cancer risk: evidence from human population studies. Nutrition and cancer, 50(1), 1-7.

Obinaju B.E., (2021). Evaluation of arsenic toxicity and its role in carcinogenicity. International Journal of Public Health and Epidemiology.10(1); 001 - 006

Oyewo, E. B., Afolabi, O. K., Oyewo, B. T., and Akanji, A. M. (2017). Ameliorative potentials of yoyo bitters and aqueous leaf extract of Moringa oleifera in arsenite-induced inflammatory dysfunctions in male Wistar rats. Asian Journal of Medicine and Health, 7(4): 1-15.

Oyewo, E.B, Adeleke, G. E, Ajayi, J. O, Adedeji A. L, Fatoki, J. O, and Oyewo B.T., (2021). Patterns of Some Immunological Proteins and Oxidative Indices in Quercetin Treated Normal and High Body Mass Index Arsenic Administered Male Wistar Rats. Himalayan Journal Applied Medical Sciences, 2(5), 106-125.

Oyewole O.I., Akinbamijo T.O., Omoboriowo O.J., Ukana N.U., (2015). Hepatorenal Protective Activities of Plantain Root (Musa paradisiaca) on Arsenic-induced Oxidative Damage in Rat. Advances in Biochemistry. Vol. 3, No. 2, pp. 30-34.

Reitman S., Frankel S., (1957). A colorimetric method for the determination of serum glutamic oxalo acetic and glutamic pyruvic transaminases. America Journal Clinical Pathology.;28:56–63.

Sampson, L., Rimm, E., Hollman, P. C., de VRIES, J. H., & Katan, M. B. (2002). Flavonol and flavone intakes in US health professionals. Journal of the American Dietetic Association, 102(10), 1414 - 1420.

Singh N, Kumar D, Lal K, Raisuddin S, Sahu AP. (2010). Adverse health effects due to arsenic exposure: Modification by dietary supplementation of jaggery in mice. Toxicol Appl Pharmacol. 242: 247–255.

Singh, M. K., Yadav, S. S., Gupta, V., and Khattri, S., (2013). Immunomodulatory role of Emblica officinalis in arsenic-induced oxidative damage and apoptosis in thymocytes of mice. BMC Complementary and Alternative Medicine, 13, 193193.

Steinmaus C., Castriota F., Ferreccio C., Smith A. H., Yuan Y., Liaw J., Acevedo J., Pérez L., Meza R., Calcagno S., Uauy R., (2015). Obesity and excess weight in early adulthood and high risks of arsenic-related cancer in later life. Environmental Research.142:594-601.

Wu S., (2021). Protective effects of curcumin on ATO-induced nephrotoxicity in ducks about suppressed autophagy, apoptosis and dyslipidemia by regulating oxidative stress. Ecotoxicol. Environ. Saf.; 142:94-105.

Xian X, Liang C, Sheng J, Yan S, Huang K, Li Z., (2017). Association between serum arsenic levels and gestational diabetes mellitus: A population-based birth cohort study. Environ Pollut. 235:850–6.

Yueshui Z., Bo C., Jing S., Lin W., Yinxin Z., Tao Y., and Zhangang X., (2017). The Beneficial Effects of Quercetin, Curcumin, and Resveratrol in Obesity. Oxidative Medicine and Cellular Longevity; 1459-1497

Zhang W., Zheng Y., Yan F., Dong M., Ren Y., (2023). Research progress of quercetin in cardiovascular disease. Front Cardiovasc Med. 16 (10):1203713.

Downloads

Published

2024-08-23

How to Cite

Synergistic Ameliorative Capabilities of Quercetin and Ascorbic Acid on Hepatic and Pro-Inflammatory Markers of Arsenic-Induced Toxicity in Obese Wistar Rats. (2024). Fountain Journal of Natural and Applied Sciences, 13(2). https://doi.org/10.53704/fujnas.v13i2.509