Partial Purification of Amylase from the Culture Filtrates of Aspergillus flavus Grown on Cassava Peels

Main Article Content

M. O. Oyewale

Abstract

Amylase from a Cassava peel culture of Aspergillus flavus was partially purified by Ammonium Sulphate precipitation as well as dialysis. The dialysed 60% (NH4)2.SO4 precipitated enzyme had an activity of 1.087 mg T.R.S released/ min/mg protein which was two folds of the activity of the crude culture filtrate. Hydrogen ion concentration as well as temperature had profound influence on enzyme activity of the partially purified enzyme while Amylase activity increased progressively as pH was increased from 3 to 7 reaching a maximum of 1.68 mg T.R.S released/min/mg/protein at PH 7.0. A rapid decrease in amylase activity was observed as pH was increased from 7 to 9 while the amylase activity increased with increase in temperature from 30 oC to 45 oC and reached a maximum of 1.15 mg T.R.S. released/min/mg/protein at 45 oC. Subsequent increase in temperature resulted into decrease in activity of the amylase enzyme.


Keywords: Aspergillus flavus; dialysis; pH; temperature; partial purification; cassava peel

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Article Details

How to Cite
Oyewale, M. O. (2012). Partial Purification of Amylase from the Culture Filtrates of Aspergillus flavus Grown on Cassava Peels. Fountain Journal of Natural and Applied Sciences, 1(1). https://doi.org/10.53704/fujnas.v1i1.57
Section
Articles

References

Akinyosoye, F. A. and Akinyanju, J. A. (1989). Effects of different Carbon sources on growth and sporulation of Geotrichum candidum. Nigerian Journal of Botany 2 , 89-95.

Bernfield, P. (1955). In method in Enzymology (Edited by colowick, S.P. Kapan, N.O) Academic press New-York, Vol. 1,149-158.

Chapman, E. S. Evans, E. Jacobeli, M. C. logana, A. (1975). The cellulolytic and Amylolytic activity of Papulaspora T hermophil Mycologia 67: 608-615.

Demot, R and Verachtert, H. C. (1987). Purification and characterization of extracellular amylase and glucoamylase from yeast Candidaantrctica. CBS. 6678 . European Journal of Biochemistry 164, 643654.

Ettalibi, M. and Baratti, J. A. (1988). Isolation and characterization of an amylolytic yeast Candidaedax. Mircen Journal of Applied Microbiology and Biotechnology 4, 193-202.

Fergus, C. L. (1969). Production of amylase by some thermophillic fungi. Mycologia 61 , 1171-1175.

Kelly, C. T. and Fogarthy, W. M. (1976). Microbial Alkaline enzymes. Process Biochemistry 11, 39.

Mohamed, Lagzouli, Mennane Zakaria, Artounejjar Ali, Wafae Senhaji, Ouhssine Mohamed, Elyachioui Mohamed, Berny El-Hassan and Jadal Mohamed, (2007). Optimization of growth and extracellular glucoamylase production by candida famata isolate. African Journal of Biotechnology 6(22), 2590-2595.

Ogundero, V. W. (1979). Amylase and cellulase activities of thermophilic fungi causing deterioration of Tobacco products in Nigeria. Mycopthologia 69 (3), 131-135.

Ogundero, V. W. (1982a). The production and activity of hydrolytic exoenzymes by Toxigenic species of Aspergillus from Garri. Nigerian Journal of Science 16, 11 - 20.

Ogundero, V. W. (1982b). Cellulose hydrolysis by thermophilic fungi from composting plant materials. Nigerian Journal of Science 16(2), 83-93.

Ogundero V. W. and Osunlaja, S. O. (1986). Glucoamylase production and activity by Aspergillus clavatus from malting barley. Mycopathologia 96, 153-156.

Oyewale, M. O. (2009). Optimization of growth and Amylase Production by Aspergillus flavus grown on some selected food and waste in Nigeria. 33rd Annual Conference and General Meeting, Nigerian Society for Microbiology, held at Osun State University between 22nd 29th November 2009.

Oyewale M. O. (2010). Growth and Amylase production by Aspergillus flavus grown on Cassava peel and commercial Soluble starch . Journal o f Pure and Applied Science 7(1&2), 53-56.

Oyewale, M. O. (2012). Optimization of Growth and Amylase production by Aspergillus flavus Grown on some Agricultural Raw matereials in Nigeria. Pakistan journal of Nutrition 11. (Accepted for Publication).

Pestana, F. and Castillo, F. J. (1985). Glucoamylase production by Aspergillus awamori on rice flour medium and partial characterization of the enzyme. Mircen Journal of Applied Microbiology and Biotechnology 1. 225-237.

Rai. M. K. and Deshmukh S. K. (2005). fungi Diversity and Biotechnology, scientific publishers, India 510 pages.

Rochi-chui-yu and Hang, Y. D. (1990). Amylolytic enzymes production by Rhizopus oryzae grown on agricultural commodities. World Journal of Microbiology and Biotechnology 6, 15-18 Sanjeer Kumar and T. Satynarayana (2005). Starch hydrolyzing enzymes of thermaphilic moulds. In Fungi diversity and Biotechnology, MK. Rai and S.K Deshmukh Eds. Scientific publishers, (Indian) pg. 229-250). Ueda, S., Saha, B. C. and Koba, Y. (1984). Direct hydrolysis of raw starch. Microbiological S ciences 1(1), 21-24.