Effects of Aqueous Root Extract of Jatropha curcas on Hyperglycaemic and Haematological Indices in Alloxan-induced Diabetic Rats
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Abstract
Hypoglycaemic potentials of aqueous root extract of Jatropha curcas (250 and 450 mg/kg body weight of rats) was investigated in alloxan-induced diabetic rats. Effect of the extract on some haematological parameters was also investigated. Thirty albino rats (Rattus norvegicus) of mean weight 165.0 ± 2.50 g were randomly selected into normal and diabetic groups, each with six rats. Diabetic treated rats were placed on 14.2 mg/kg body weight glucophage (reference drug), 250 mg/kg and 450 mg/kg body weight of extract. All treatments were administered orally, once daily for fifteen days. The blood glucose levels were monitored and the haematological parameters like haemoglobin (Hgb), red blood cell (RBC) count, white blood cell (WBC) count, packed cell volume (PCV), percentage lymphocytes (LYM) and neutrophils (NEUT) were also analyzed. A significant (p<0.05) reduction in fasting Blood Glucose Levels (BGL) was observed in alloxan-induced diabetic rats treated with the extract. There was a significant (p<0.05) reduction in Hgb, PCV, RBC and WBC and its differentials of the diabetic untreated rats while administration of glucophage, 250 and 450 mg/kg body weight of the aqueous extract (Jc250 and Jc450) to diabetic rats significantly (p<0.05) increase all these parameters. These results suggested that the aqueous root extract of Jatropha curcas possesses hypoglycaemic activity and also improved the aberrations in the blood parameters of alloxan-induced diabetic rat.
Keywords: Jatropha curcas, root, aqueous extract, diabetic rats, hypoglycaemic, haematology
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References
Achten, W. M. J., Verchot, L., Franken, Y. J., Mathijs, E., Singh, V. P., Aerts, R. and Muys, B. (2008). Jatropha biodiesel production and use (a review). Biomass and Bioenergy, 32 (2): 1063-1084.
ADA (2010). America Diabetes Association Diabetes care 33, S1 S62-69.
Anaja, H. P. (1995). Relation of plasma fructosamine in lipid profiles in diabetic patients. International Diabetes Digest, 6: 64-66.
Andreoli, T. E., Carpenter, C. C. Plum, F. and Smith, L. H. (1990). Diabetes mellitus. In: Cecil Essential of Medicine, J. Dyson, (Ed.)., 2nd Edn., WB Saunders, Philadelphia, 1: 496505.
Ashafa, O. T., Yakubu, M. T., Grierson, D. S. and Afolayan, A. J. (2009). Toxicological evaluation of the aqueous extract of Felicia muricata Thunb. leaves in Wistar rats. African Journal of Biotechnology 8:949-54.
Burkill, H. M. (1994). The Useful Plants of West Tropical Africa. Vol. 2, Royal Botanical Gardens, Kew, UK.
Dacie, J. C and Lewis, S. (1991). Practical Hematology, 7th edition , Churchill Livingstone, New York pp.
Dehygan, B. and Webster, G. L. (1997). Morphology and intragenic relationship of the Genus Jatropha. University of Califfornia publications in Botany, vol. 74.
Diabetes Care January (2010). vol. 33 no. Supplement 1 S62-S69.
Djrolo, F., Houngbe, H., Avode, G., Addra, B., Kodjoh, N., Avinadje, M. and Monterio, B. (1998). Malnutrition related diabetes ( tropical diabetes). Med. Afrique Noire, 45: 538-542.
Fojas, F. R., Garia, L. L., Venzon, E. L., Sison, F. M., Villamiera, B. A., Jojas, A. J. and Liava, I. (1986). Pharmaceutical studies of Jatropha curcas as a possible source of antiarrhythmic (beta blocker) agent. Phillipp. J. Sci. 115:317-328.
Gadekar, K. P. (2006). Vegetative propagation of Jatropha curcas, Karanji and Mahua by stem cuttings,Grafting, Budding and Air-layering, M.Sc. Forestry Thesis, Department of Forestry, IndraGandhi Agricultural University, Raipur.
Gonasekera, M. M., Gunawardan, V. K., Jayasena, K., Mohammed, S. G. and Balasubramania, S.
(1995). Pregnancy terminating effects of Jatropha curcas in rats. Journal of Ethnopharmacology, 47 (3): 117-123.
Grover, J. K, Yadav, S. and Vats, V. (2002). Medicinal plants of India with hypoglycemic potentials. Journal of Ethnopharmacol. 81: 81-100.
Guthrie, D. W. and Guthrie, R. A. (2002). Nursing management of diabetes mellitus: A guide to the pattern approach. 5th ed. New York: Springer.
Holman, R. R. and R. C. Turner. (1991). Oral Agents and Insulin in the Treatment of NIDDM. In: Textbook of Diabetes. P.S. Williams, (Ed.). Blackwell, Oxford, pp: 467-469.
Harborne, J. B. (1973). Phytochemical methods; A guide to modern techniques of plant analysis. Chapman and Hall, London, p. 279
Igoli, J.O., Ogaji, D. G., Tor Anyim, T. A. and Igoli, N. P. (2005). Traditional Medicine practice among the Igede people of Nigeria. African J. Traditional Complimentary and Alternative Medicine, 2 (2): 134-152.
Ivorra, M. D., Paya M., Villar A. (1989). A review of natural products and plants as potential anti-diabetic agents. J. Ethnopharmacol. 27:243 -275.
Kakade, M. L., Simons, N. R., Liener, I. E. and Lambert, J.W. (1972). Biochemical and nutritional assessment of different varieties of soybeans. J. Agric. Food Chem. 20:87-90.
Kako, M., Miura, T., Nishiyama, Y., Ichimaru, M., Mariyasu, M. and Kato, A. (1997).Hypoglycemic activity of some Triterpenoid Glycosides. J. Nat. Prod. 60: 604-605.
Kamtchoung, P., Sokeng, D. S., Moundipa, F. P., Watcho, P., Jatsa, B. H. and Lontsi, D. (1998) Protective role of Anarcardium occidentale extract against streptozotocin-induced diabetes in rats. J. Ethnopharmacol.; 62:55-99. Kim, H.Y., Moon, B.H., Lee, H.J and Choi, D.H. (2004). Flavonoids glycosides from the leaves of Eucommia ulmoides with glycation inhibitory activity. J. Ethnopharmacol. 93: 227-230.
Larner, J. (1985). Insulin and Oral Hypoglyceamic Drugs: Glucagon. In: Pharmacological Basis of Therapeutics, A.G. Gilman, L.S. Goodman and A. Gilman (Eds.)., 7th Edn., Macmillan, London, pp: 1490-1516.
Mansi, K. M. S. (2006). Effects of administration of alpha-melanocyte stimulating hormone (± MSH) on some hematological values of alloxaninduced diabetic rats. Am. J. Pharmacol.
Toxicol.1:5-10.
Marles, R and Farnsworth, N. (1995). Antidiabetic plants and their active constituents. Phytomedicine 2:137-165.
Moss, P.P. (1999). Blood banking: concepts and application pp 12-34 Philadelphia, P. A. Saunder.
Mohammed, A., Adelaiye, A.B., Bakari, A.G. and Mabrouk, M.A. (2009). Anti-diabetic and some haematological effects of ethylacetate and nbutanol fractions of Ganoderma lucidum aqueous extract in alloxan-induced diabetic Wisar rats. Int. J. Med. Sci. 1:530-5.
Muhammad, N.O., Adeyina, A.O and Peters, O.M. (2000). Nutritional evaluation of fungi trerated cocoa bean shell. Nigerian J. Pure Appl. Sci. 5: 1059-64.
Muhammad, N.O. and Oloyede O.B. (2009). Haematological parameters of broiler chicks fed Aspergillus niger-fermented Terminalia catappa seed meal-based diet. Global J. Biotechnol. Biochem. 4:179-183.
Nabel, E.G. (2003). Cardiovascular disease. N. Eng. J. Med., 349:60-72.
Nagappa, A. N., Thakurdesai P.A. Venkat Rao, N and Jiwan, S. (2003). Anti-diabetic activity of
Terminalia catappa Linn. Fruits. J. Ethnopharmacology 88: 45-50.
Nojima, H., Kimura, I. and Chen, F.J. (1998). Antihyperglycemic effects of N-containing sugars from Xanthocercis zambesiaca, Morus bombycis, Aglaonema trubii and Castanosperrmin australe in STZ-diabetic mice. J. Nat. Prod. 61: 397-400.
Odebiyi, O.O. and Sofowara, E. A. (1980). Phytochemical screening of Nigerian medicinal plants. 2nd OAU/STRC Inter-African Symposium on Traditional Pharmacopoeia and African medicinal plants. Lagos 115:216220.
Okokon, J.E., Ita, B.N. and Udokpoh, A.E. (2006). The in-vivo Anti-malarial activities of Uvariae chamae and Hippocratea africana. Ann. Trop.Med. Parasit. 100(9):585. 590.
Pari, L. and Amarnath, S. (2004). Antidiabetic activity of Boerhavia diffusa L. effect on hepatic key enzymes in experimental diabetic rats. J. Ethnopharmacol. 91: 109-113.
Reher, G., Slijepcevic, M. and Krans, L. (1991). Hypoglycaemic activity of triterpenes and tannins from Sarcopoterium spinosum and two Sanguisorba Species. Planta Med. 57: A57-A58.
Sharma, A. K. (ed) (1993). Diabetes mellitus and its complications: an update. New Delhi: Macmillan India Ltd, (pp 92-205).
Shimizu, M., Ito, T., Terashima, S., Mayashi, T., Arisawa, M., Morita, N., Kurokowa, S., Ito, K. and Hasimato,Y. (1984). Inhibition of lens aldolase reductase by flavonoids. Phytochemistry. 23: 1885 -1888.
Szkudelski, T. (2001). The mechanism of Alloxan and Streptozocin action in I²-cell of the rats' pancreas. Physiol Res. 50: 536-546.
Thomas, O.O. (1989). Re-examination of the antimicrobial activities of Xylopia aethiopica, Carica papaya, Ocimum gratissimum and Jatropha curcas, Fitoterapia, vol. 60, no. 2, pp. 147-155.
Trease, G.E. and Evans, W.C. (1989). A Textbook of Pharmacognosy 13th ed.376-389.
Wall, M.E. Eddy, C.R., Mc Clenna, M.I. and Klump, M.E. (1952).Detection and estimation of steroid sapogenins in plant tissues. Anal. Chem. 24,1337
Wall, M. E.,Krider, M. M., Krewson, C. F., Eddy, C. R., William, J. J., Cordell, D. S. and Gentry, H.S. (1954). Steroidal sapogenins XIII. Supplementary table of data for steroidal sapogeninsVII. Agr. Research Service Circ. Aic, 363,pp17.
WHO (1980). Expert committee on diabetes mellitus. Tech. Rep. Series No. 646. World Health Organization, Geneva.
WHO (2002). WHO launches the first global strategy on traditional medicine: Press release WHO p.38.
Williams, G. and Pickup, J.C. (1991). New Drugs in the Management of Diabetes Mellitus. In: Textbook of Diabetes, Pickup, J.C. and G. Williams (Eds.). Blackwell, Oxford, 11: 977-993. Yakubu, M.T., Akanji, M.A. and Oladiji, A.T. (2007). Haematological evaluation in male albino rats following chronic consumption of aqueous extract of Fadogia agrestis stem. Pharmacol Manag 3:34-38.
Zarzuelo, A., Jimenez, I. and Gamez, M.J. (1996). Effects of luteolin 5-O-betarutenoside in STZ-induced Diabetic rats. Life Sci. 58: 23112316.
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