Antimicrobial Activities of Bryophyllum pinnatum on Some Selected Clinical Isolates.

  • I A Ibikunle University of Ilorin, P.M.B. 1515, Ilorin, Kwara State, Nigeria

Abstract

Ethanol, methanol and aqueous extracts of the leaves of Bryophyllum pinnatum obtained through cold maceration, were screened for their antibacterial activities against selected multi-drug resistant bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi Staphylococcus aureus) using the agar well diffusion method. Broth dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts at concentrations ranging from 100mg/ml to 25mg/ml. The ethanol extract was the most reactive while the aqueous extract showed lesser antibacterial activity. Ofloxacin was the most effective antibiotic in the antibiotic susceptibility profiling of the test organisms. It was however evident that the ethanol extract of Bryophyllum pinnatum has higher antibacterial efficacy on the test organisms than Ofloxacin. Bacteriostatic and -cidal activities were exhibited by the plant extracts against the organisms ranging from 25 – 100 × 103 µg/ml. Succinctly, Bryophyllum pinnatum possesses biologically active constituents with explorable pharmacological potentials.

Author Biography

I A Ibikunle, University of Ilorin, P.M.B. 1515, Ilorin, Kwara State, Nigeria
Department of Microbiology, Faculty of Life Sciences.

References

Adesanya, S. A. (2005). Theories and realities. Inaugural lecture series 181,Published by Obafemi Awolowo University Press Ltd., Ile-Ife, Osun State, Nigeria.

Aibinu, I., Adenipekun, E. and Odugbemi, T. (2004). Emergence of Quinolone Resistance amongst Escherichia coli strains isolated from clinical infections in some Lagos State Hospitals in Nigeria. Nigerian Journal of Health and Biomedical Science. 3(2):73-78.

Akinpelu, D.A. (2000). Antimicrobial activity of Bryophyllum pinnatum leaves. Fitoterapia 71:193-194.

Akinyemi, K. O., Oladapo, O., Okwara ,C.E., Ibe ,C.C. and Fasure, K.A. (2005). Screening of crude extracts of six medicinal plants used in Southwest Nigerian unorthodox medicine for antimethicillin resistant Staphylococcus aureus activity. BMC Complementary and Alternative Medicine. 5:6-7

Anibijuwon, I., Gbala, I., Adeyemi, J. and Abioye, J. (2017). Antibacterial activity of stingless bee (Dactylurina studingeri) propolis on bacteria isolated from wound. SMU Medical Journal 4(1): 43-50.

Aquil, F. and Ahmad, I. (2003). Broad-spectrum antibacterial and antifungal properties of certain traditionally used Indian medicinal plants. World Journal Microbiology. Biotechnology. 19:653-657.

Calixto, J. B. (2000). Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicine (phytotherapeutic agents). Brazilian Journal of Medical and Biological Research. 33:179-189.

Centre for Disease and Control (CDC) (2014). Risk assessment on the spread of carbapenemase-producing Enterobacteriaceae (CPE). www.cdc.gov/eid
Clinical and Laboratory Standards Institute (CLSI) (2016). Performance Standards for Antimicrobial Susceptibility Testing. 26th ed. CLSI supplement M100S. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500,
Wayne, Pennsylvania 19087 USA ISBN 1-56238-924-6 [Electronic]

Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Review. 12: 564-582.

Gills, L.S. (1992). The ethnomedical uses of plants in Nigeria. Published by University of Benin Press, University of Benin, Benin city, Edo State. Pp 46, 143.

Iwu, M.M. (1993). Hand-book of African Medicinal Plants. Published by CRC Press

Nguelefack, T.B., Sonita, B., Alain, B.D., Dimo,T., Kamanyi,A., and Vierling, W (2006). Spasmolytic effects of extracts from kalanchoe crenata leaves. Pharmacology. 1:30-39.

Obaseiki-ebor, E.E. (1985): Preliminary Report on the in-vivo Antibacterial Activity of B. pinnatum Leaf Juice. African Journal Medical Science. 14(34).199-202.

Ofokansi, K.C., Esimone, C.O. and Anele, C.R. (2005). Plant Products Research Journal. 9: 23-27

Ojewole, J.A. (2005). Antinociceptive, Antiinflammatory and Antidiabetic Effects of B. pinnatum (Crassulaceae) Leaf aqueus Extract. Journal of Enthnopharmacol. 13; 99(1):13-9.

Okwu, D. E. and Josiah, C. (2006). Evaluation of the chemical composition of two Nigerian medicinal plants. African Journal of Biotechnology 5 (4):257-361.

Oliver-Bever, B. (1989). Medicinal plants in tropical West Africa. Cambridge University Press. Pp 20, 35.

Raskin, I., Ribnicky, D.M, Komarnytsky, S., Ilic, N., Pouley A. and Borisjuk N. (2002). Plants and human health in the twenty-first century. Trends of Biotechnology.20:522-531.

Sofowora, A., (1993). Medical plants and traditional medicine in Africa. Published by Spectrum books Ltd. Ibadan.

Sofowora, S.A. (1986). Medicinal plants and Traditional Medicine in Africa. Spectrum books Ltd. 1st edition. 150-151 and 162-172.

Theophil, D., Agatha, F.L., Nguelefack, T.B., Asongalem, E.A. and Kamtchouing, P. (2006). Anti-inflammatory activity of leaf extracts of Kalanchoe crenata. Indian Journal of Pharmaceuticals. 38:115-117.

Williams R. (2000) Antimicrobial resistance a global threat. Essential Drug Monitor.28–29:1.
How to Cite
Ibikunle, I. (1). Antimicrobial Activities of Bryophyllum pinnatum on Some Selected Clinical Isolates. Fountain Journal of Natural and Applied Sciences, 6(1). Retrieved from http://fountainjournals.com/index.php/FUJNAS/article/view/135